How reliable are experimental protein-protein interaction data? | Laboratory of Professor Hanah Margalit

How reliable are experimental protein-protein interaction data?

Citation:

Sprinzak E, Sattath S, Margalit H. How reliable are experimental protein-protein interaction data?. JOURNAL OF MOLECULAR BIOLOGY. 2003;327:919-923. Available at: https://doi.org/10.1016/S0022-2836(03)00239-0.

Date Published:

APR 11

Abstract:

Data of protein-protein interactions provide valuable insight into the molecular networks underlying a living cell. However, their accuracy is often questioned, calling for a rigorous assessment of their reliability. The computation offered here provides an intelligible mean to assess directly the rate of true positives in a data set of experimentally determined interacting protein pairs. We show that the reliability of high-throughput yeast two-hybrid assays is about 50%, and that the size of the yeast interactome is estimated to be 10,000-16,600 interactions. (C) 2003 Elsevier Science Ltd. All rights reserved.

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DOI:

10.1016/S0022-2836(03)00239-0