@article { WOS:000231516900028,
	title = {Assigning functions to genes: identification of S-phase expressed genes in Leishmania major based on post-transcriptional control elements},
	journal = {NUCLEIC ACIDS RESEARCH},
	volume = {33},
	number = {13},
	year = {2005},
	pages = {4235-4242},
	publisher = {OXFORD UNIV PRESS},
	type = {Article},
	address = {GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND},
	abstract = {Assigning functions to genes is one of the major challenges of the post-genomic era. Usually, functions are assigned based on similarity of the coding sequences to sequences of known genes, or by identification of transcriptional cis-regulatory elements that are known to be associated with specific pathways or conditions. In trypanosomatids, where regulation of gene expression takes place mainly at the post-transcriptional level, new approaches for function assignment are needed. Here we demonstrate the identification of novel S-phase expressed genes in Leishmania major, based on a post-transcriptional control element that was recognized in Crithidia fasciculata as involved in the cell cycle-dependent expression of several nuclear and mitochondrial S-phase expressed genes. Hypothesizing that a similar regulatory mechanism is manifested in L.major, we have applied a computational search for similar control elements in the genome of L.major. Our computational scan yielded 132 genes, of which 33\% are homologues of known DNA metabolism genes and 63\% lack any annotation. Experimental testing of seven of these genes revealed that their mRNAs cycle throughout the cell cycle, reaching a maximum level during S-phase or just prior to it. It is suggested that screening for post-transcriptional control elements associated with a specific function provides an efficient method for assigning functions to trypanosomatid genes.},
	issn = {0305-1048},
	doi = {10.1093/nar/gki742},
	url = {https://doi.org/10.1093/nar/gki742},
	author = {Zick, A and Onn, I and Bezalel, R and Margalit, H and Shlomai, J}
}
