@article { WOS:000351638000011,
	title = {A defense-offense multi-layered regulatory switch in a pathogenic bacterium},
	journal = {NUCLEIC ACIDS RESEARCH},
	volume = {43},
	number = {3},
	year = {2015},
	month = {FEB 18},
	pages = {1357-1369},
	publisher = {OXFORD UNIV PRESS},
	type = {Article},
	address = {GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND},
	abstract = {Cells adapt to environmental changes by efficiently adjusting gene expression programs. Staphylococcus aureus, an opportunistic pathogenic bacterium, switches between defensive and offensive modes in response to quorum sensing signal. We identified and studied the structural characteristics and dynamic properties of the core regulatory circuit governing this switch by deterministic and stochastic computational methods, as well as experimentally. This module, termed here Double Selector Switch (DSS), comprises the RNA regulator RNAIII and the transcription factor Rot, defining a double-layered switch involving both transcriptional and post-transcriptional regulations. It coordinates the inverse expression of two sets of target genes, immuno-modulators and exotoxins, expressed during the defensive and offensive modes, respectively. Our computational and experimental analyses show that the DSS guarantees fine-tuned coordination of the inverse expression of its two gene sets, tight regulation, and filtering of noisy signals. We also identified variants of this circuit in other bacterial systems, suggesting it is used as a molecular switch in various cellular contexts and offering its use as a template for an effective switching device in synthetic biology studies.},
	issn = {0305-1048},
	doi = {10.1093/nar/gkv001},
	url = {https://doi.org/10.1093/nar/gkv001},
	author = {Nitzan, Mor and Fechter, Pierre and Peer, Asaf and Altuvia, Yael and Bronesky, Delphine and Vandenesch, Fran{\c c}ois and Romby, Pascale and Biham, Ofer and Margalit, Hanah}
}
