@article { WOS:000417649500005,
	title = {Post-transcriptional 3{\textquoteright}-UTR cleavage of mRNA transcripts generates thousands of stable uncapped autonomous RNA fragments},
	journal = {NATURE COMMUNICATIONS},
	volume = {8},
	year = {2017},
	month = {DEC 11},
	pages = {2029},
	publisher = {NATURE RESEARCH},
	type = {Article},
	address = {HEIDELBERGER PLATZ 3, BERLIN, 14197, GERMANY},
	abstract = {The majority of mammalian genes contain one or more alternative polyadenylation sites. Choice of polyadenylation sites was suggested as one of the underlying mechanisms for generating longer/shorter transcript isoforms. Here, we demonstrate that mature mRNA transcripts can undergo additional cleavage and polyadenylation at a proximal internal site in the 3{\textquoteright}-UTR, resulting in two stable, autonomous, RNA fragments: a coding sequence with a shorter 3{\textquoteright}-UTR (body) and an uncapped 3{\textquoteright}-UTR sequence downstream of the cleavage point (tail). Analyses of the human transcriptome has revealed thousands of such cleavage positions, suggesting a widespread post-transcriptional phenomenon producing thousands of stable 3{\textquoteright}-UTR RNA tails that exist alongside their transcripts of origin. By analyzing the impact of microRNAs, we observed a significantly stronger effect for microRNA regulation at the body compared to the tail fragments. Our findings open a variety of future research prospects and call for a new perspective on 3{\textquoteright}-UTR-dependent gene regulation.},
	issn = {2041-1723},
	doi = {10.1038/s41467-017-02099-7},
	url = {https://doi.org/10.1038/s41467-017-02099-7},
	author = {Malka, Yuval and Steiman-Shimony, Avital and Rosenthal, Eran and Argaman, Liron and Cohen-Daniel, Leonor and Arbib, Eliran and Margalit, Hanah and Kaplan, Tommy and Berger, Michael}
}
