@article { WOS:001273306100001,
	title = {Unraveling the interplay between a small RNA and RNase E in bacteria},
	journal = {NUCLEIC ACIDS RESEARCH},
	volume = {52},
	number = {15},
	year = {2024},
	month = {JUL 22},
	pages = {8947-8966},
	publisher = {OXFORD UNIV PRESS},
	type = {Article},
	address = {GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND},
	abstract = {Small RNAs (sRNAs) are major regulators of gene expression in bacteria, exerting their regulation primarily via base pairing with their target transcripts and modulating translation. Accumulating evidence suggest that sRNAs can also affect the stability of their target transcripts by altering their accessibility to endoribonucleases. Yet, the effects of sRNAs on transcript stability and the mechanisms underlying them have not been studied in wide scale. Here we employ large-scale RNA-seq-based methodologies in the model bacterium Escherichia coli to quantitatively study the functional interaction between a sRNA and an endoribonuclease in regulating gene expression, using the well-established sRNA, GcvB, and the major endoribonuclease, RNase E. Studying single and double mutants of gcvB and rne and analysing their RNA-seq results by the Double Mutant Cycle approach, we infer distinct modes of the interplay between GcvB and RNase E. Transcriptome-wide mapping of RNase E cleavage sites provides further support to the results of the RNA-seq analysis, identifying cleavage sites in targets in which the functional interaction between GcvB and RNase E is evident. Together, our results indicate that the most dominant mode of GcvB-RNase E functional interaction is GcvB enhancement of RNase E cleavage, which varies in its magnitude between different targets. Graphical Abstract},
	issn = {0305-1048},
	doi = {10.1093/nar/gkae621},
	url = {https://doi.org/10.1093/nar/gkae621},
	author = {Vigoda, Meshi Barsheshet and Argaman, Liron and Kournos, Mark and Margalit, Hanah}
}
