Primers used for PCR amplification.

SraB

524 5- CGC CAT GGC GAG GTG GAG 3

525 5 GCA TAG GGC GCG CAT AT 3

sraC

501 5- TCC TCC CGC GCT AAA GA - 3

502 5 CTC TGT TAA TTA CCT AA - 3

sraE

532 5 TTC GGG TTG AGC ACA TG 3

522 5 AAA GCG AAT GGA GGG AG 3

sraF

511 5 TTG CCA AAG TAA AAC AGT G 3

512 5 GGC GAC AAC AAC AGC GA 3

sraH

503 5 - CGC AAT TGA CTG AAA CAC 3

504 5 - AGT GGC GTC ACG ACG TA 3

sraI

509 5 GTC CCT TTC AAC ATC ATT G 3

510 5 GGA GTG CGC ATA ACG AA 3

sraJ

505 5 GTA TGT GTA GGA TCA AGC 3

506 5 GCC ACA GTA GTT CCT TC 3

sraL

507 5 GTC GGT GCG CAA GAT TG 3

508 5 GCC CGT TGC GTA ACC CG 3

rprA

513 5 GGC GAA ATC GAG AAA CC 3

514 5 GCC TGC TGA CGG CTT GA 3

Primers for primer extension assays

sraB

495 5 TGC CAA TAA TTC GCA CA 3

496 5 AAC CGG ATC GAG AGT CA 3

sraC

449 5 TCA GCT GAT GAC CAC CA 3

541 5 TCC CTG GAC CGA ATA CA 3

sraD

534 5 GAT AAC AAA TGC GCG TC 3

sraE

542 5 GTA CCG AAT AAT CTC ACC AA 3

sraG

539 5 AGG GTT GTC ATT AGT GC 3

sraH

448 5 CGA ATA CTG CGC CAA C 3

sraI

470 5 CTG GAA GCA ATG TGA GC 3

sraJ

480 5 CAC TCA ACG TTG TGT CC 3

sraK

483 5 CTG ACC CTC ATC CTG AG

sraL

  1. 5 TTT CTC TCT ATC CCG CTG 3

rprA

  1. 5 CAA AGA CTA CAC ACA GCA A 3

Primers for 5 and 3 RACE experiments

sraB

5 RACE

149-A 5 CAC ACA TTG CGG GT- 3

149-B 5 TGC GGT TTA CTG CTT TAT 3

3 RACE

149-C 5 GTT ATC AGC ACT GAA CGA AAA 3

149-D 5 GCA GTA ACC CGC AAT GT 3

149-E 5 TCC ATC AGA TAT AGC GTA TTG A 3

149-F 5 TAG CGT ATT GAT GAT AGC CAT 3

sraC

5 RACE

101 5 CAG CTG ATG ACC ACC A 3

101-B 5 TGA TGA CCA CCA CGC TT- 3

3 RACE

101-A 5 TGG TCA TCA GCT G 3

101-C 5 GGT CAT CAG CTG AAA TGT 3

101-D 5 GTC AGC GAA GGA AAT GCT 3

101-E 5 GAG ACC GAA CAC GAT TCC 3

101-F 5 GCC TTG CCC TTT AAG AAT AG 3

sraD

5 RACE

150-A 5 GGA ATT CAG GGA TGA TG 3

150-B 5 GCG CGT CTT TCA TAT ACT CA 3

150-C 5 TCA GGG ATG ATG ATA ACA AAT GCG 3

150-D 5 TTC ATC TCT GGA ATT CAG GGA T 3

sraE

5 RACE

4-A 5 GTA CCG AAT AAT CTC ACC AA 3

3 RACE

4-B 5 TTG GTG AGA TTA TTC GGT AC 3

SraF

5 RACE

109-C 5 CCG CTG CTT TTT ATG 3

109-D 5 TAT GCA TAG ACC TCG CCT T 3

109-E 5 GGT GAC CGG ATG TGT TT 3

3 RACE

109-A 5 GTG AGA CCT TGC CGG A 3

109-B 5 GCC GGA AGG CGA G 3

109-F 5 GCC GGT CGA TCG TC 3

sraG

5 RACE

444-A 5 ATA GAG CTG GGT TAG GG 3

444-B 5 GGG TTA GGG TTG TCA TTA GTC 3

444-C 5 - CTG ACG GCA GCA ATT 3

444-D 5 GCA ATT TAC TGG AAA CTA ATG 3

3 RACE

444-E 5 GCA TCC TCG CGA CTA AT 3

444-F 5 GAA CCT CTG CAA CGG AA 3

444-G 5 GCT GCC GTC AGC TTG 3

sraH

5 RACE

102 5 CGA ATA CTG CGC CAA 3

102-B 5 GAA TAC TGC GCC AAC AC 3

3 RACE

102-A 5 TTG GCG CAG TAT TCG 3

102-C 5 GAT TTC CCT GGT GTT G 3

102-D 5 GCT GTG CCC TTG TAA CTC 3

sraI

5 RACE

107 5 TGG AAG CAA TGT GAG C 3

107-B 5 GCA ATG TGA GCA ATG TCG 3

3 RACE

107-A 5 GCT CAC ATT GCT TCC A 3

107-C 5 TCA CAT TGC TTC CAG TAT T 3

sraJ

5 RACE

103 5 GTC AGT GGA CGA TAA GCA 3

103-B 5 GGA CGA TAA GCA CCG TAA 3

3 RACE

103-A 5 TGC TTA TCG TCC ACT GAC 3

103-C 5 GTC CAC TGA CAG ATG TCG 3

sraK

5 RACE

200 5 GCC GGA ACA ATC CGT 3

200-A 5 CGT CTC CGG ACG TTT 3

3 RACE

200-B 5 GTC CGG AGA CGT AAT TAA AC 3

sraL

5 RACE

104 5 GTT TCC CCC GAC GT 3

104-B 5 CCC GAC GTC AAC ACA C 3

3 RACE

104-A 5 ACC CTC CTG TGT ACC AG 3

104-C 5 GTA CCA GCG GGA TAG AG 3

rprA

5 RACE

100 5 GCA AAG ACT ACA CAC AGC A 3

100-A 5 CTC AGG GGA TTT CCA T 3

100-D 5 GAT GGG CAA AGA CTA CAC 3

100-E 5 GGG CAA AGA CTA CAC ACA G 3

3 RACE

100-B 5 TGC TGT GTG TAG TCT TTG C 3

100-C 5 ATG GAA ATC CCC TGA G 3

100-F 5 GAA TCT GAT CGA CGC AAA 3

 


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