Cleavage Scores


The cleavage score between amino acid residues a and b is calculated as follows:

C(a,b) = ln ( f(a,P1) / f(a) ) + ln ( f(b,P1') / f(b) )

f(a,P1) - the observed frequency of amino acid a at position P1 of a cleavage site
f(a) - the frequency of amino acid a in the protein database SWISSPROT
f(b,P1') - the observed frequency of amino acid a at position P1' of a cleavage site
f(b) - the frequency of amino acid b in the protein database SWISSPROT
The amino acid frequencies for the P1 and P1' positions were defined by analyzing the amino acids at the C-terminus and its immediate flanking residue in a database of naturally processed peptides and their source protein sequences (Altuvia & Margalit, 2000 [Pubmed abstract] )



Cleavage Scores Table

P1\P1' A C DE F GH I KL M NP Q RS T VW Y
A -0.94-0.88-2.21-1.85-3.61-1.25-2.45-1.76-0.70-1.51-1.41-1.11-1.71-1.14-0.53-0.85-1.16-1.89-1.40-1.41
C -4.77-4.70-6.03-5.68-7.44-5.08-6.28-5.59-4.53-5.34-5.24-4.94-5.54-4.97-4.36-4.68-4.99-5.72-5.23-5.24
D -2.24-2.17-3.50-3.15-4.91-2.54-3.75-3.06-1.99-2.81-2.70-2.40-3.01-2.43-1.82-2.15-2.46-3.18-2.70-2.71
E -6.11-6.05-7.38-7.03-8.78-6.42-7.62-6.94-5.87-6.69-6.58-6.28-6.89-6.31-5.70-6.02-6.34-7.06-6.58-6.59
F 1.071.14-0.190.16-1.600.76-0.440.251.320.500.600.900.300.881.481.160.850.120.610.60
G -6.18-6.12-7.45-7.10-8.86-6.49-7.70-7.01-5.94-6.76-6.65-6.35-6.96-6.38-5.77-6.10-6.41-7.13-6.65-6.66
H -1.38-1.32-2.65-2.30-4.06-1.69-2.89-2.21-1.14-1.96-1.85-1.55-2.16-1.58-0.97-1.29-1.61-2.33-1.85-1.86
I 0.770.83-0.50-0.14-1.900.46-0.74-0.051.010.200.300.60-0.000.571.180.860.55-0.180.310.30
K 0.580.64-0.69-0.34-2.100.27-0.94-0.250.820.000.110.41-0.200.380.990.660.35-0.370.110.10
L 1.291.350.020.37-1.390.98-0.230.461.530.710.821.120.511.091.701.371.060.340.820.81
M 0.840.90-0.43-0.08-1.830.53-0.670.021.080.260.370.670.060.641.250.930.61-0.110.370.36
N -5.75-5.69-7.02-6.67-8.43-6.06-7.26-6.58-5.51-6.33-6.22-5.92-6.53-5.95-5.34-5.66-5.98-6.70-6.22-6.23
P -1.15-1.09-2.42-2.07-3.83-1.46-2.67-1.98-0.91-1.73-1.62-1.32-1.93-1.35-0.74-1.07-1.38-2.10-1.62-1.63
Q -1.39-1.33-2.66-2.31-4.06-1.70-2.90-2.21-1.15-1.97-1.86-1.56-2.17-1.59-0.98-1.30-1.62-2.34-1.86-1.87
R 0.370.43-0.90-0.54-2.300.06-1.14-0.450.61-0.20-0.100.20-0.400.170.780.460.15-0.58-0.09-0.10
S -1.98-1.91-3.24-2.89-4.65-2.29-3.49-2.80-1.73-2.55-2.45-2.15-2.75-2.17-1.57-1.89-2.20-2.93-2.44-2.45
T -2.31-2.24-3.57-3.22-4.98-2.62-3.82-3.13-2.07-2.88-2.78-2.48-3.08-2.51-1.90-2.22-2.53-3.26-2.77-2.78
V 0.951.02-0.310.04-1.720.64-0.560.131.200.380.480.780.180.761.361.040.730.000.490.48
W 1.741.800.470.82-0.931.430.230.911.981.161.271.570.961.542.151.831.510.791.271.26
Y 2.122.190.861.21-0.551.820.611.302.371.551.651.951.351.932.542.211.901.181.661.65


Cleavage scores table in text format



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