The cleavage score between amino acid residues a and b is calculated as follows:
The amino acid frequencies for the P1 and P1' positions were defined by analyzing the amino acids at the C-terminus and its immediate flanking residue in a database of naturally processed peptides and their source protein sequences (Altuvia & Margalit, 2000 [Pubmed abstract] )
f(a) - the frequency of amino acid a in the protein database SWISSPROT
f(b,P1') - the observed frequency of amino acid a at position P1' of a cleavage site
f(b) - the frequency of amino acid b in the protein database SWISSPROT
| P1\P1' | A | C | D | E | F | G | H | I | K | L | M | N | P | Q | R | S | T | V | W | Y |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A | -0.94 | -0.88 | -2.21 | -1.85 | -3.61 | -1.25 | -2.45 | -1.76 | -0.70 | -1.51 | -1.41 | -1.11 | -1.71 | -1.14 | -0.53 | -0.85 | -1.16 | -1.89 | -1.40 | -1.41 |
| C | -4.77 | -4.70 | -6.03 | -5.68 | -7.44 | -5.08 | -6.28 | -5.59 | -4.53 | -5.34 | -5.24 | -4.94 | -5.54 | -4.97 | -4.36 | -4.68 | -4.99 | -5.72 | -5.23 | -5.24 |
| D | -2.24 | -2.17 | -3.50 | -3.15 | -4.91 | -2.54 | -3.75 | -3.06 | -1.99 | -2.81 | -2.70 | -2.40 | -3.01 | -2.43 | -1.82 | -2.15 | -2.46 | -3.18 | -2.70 | -2.71 |
| E | -6.11 | -6.05 | -7.38 | -7.03 | -8.78 | -6.42 | -7.62 | -6.94 | -5.87 | -6.69 | -6.58 | -6.28 | -6.89 | -6.31 | -5.70 | -6.02 | -6.34 | -7.06 | -6.58 | -6.59 |
| F | 1.07 | 1.14 | -0.19 | 0.16 | -1.60 | 0.76 | -0.44 | 0.25 | 1.32 | 0.50 | 0.60 | 0.90 | 0.30 | 0.88 | 1.48 | 1.16 | 0.85 | 0.12 | 0.61 | 0.60 |
| G | -6.18 | -6.12 | -7.45 | -7.10 | -8.86 | -6.49 | -7.70 | -7.01 | -5.94 | -6.76 | -6.65 | -6.35 | -6.96 | -6.38 | -5.77 | -6.10 | -6.41 | -7.13 | -6.65 | -6.66 |
| H | -1.38 | -1.32 | -2.65 | -2.30 | -4.06 | -1.69 | -2.89 | -2.21 | -1.14 | -1.96 | -1.85 | -1.55 | -2.16 | -1.58 | -0.97 | -1.29 | -1.61 | -2.33 | -1.85 | -1.86 |
| I | 0.77 | 0.83 | -0.50 | -0.14 | -1.90 | 0.46 | -0.74 | -0.05 | 1.01 | 0.20 | 0.30 | 0.60 | -0.00 | 0.57 | 1.18 | 0.86 | 0.55 | -0.18 | 0.31 | 0.30 |
| K | 0.58 | 0.64 | -0.69 | -0.34 | -2.10 | 0.27 | -0.94 | -0.25 | 0.82 | 0.00 | 0.11 | 0.41 | -0.20 | 0.38 | 0.99 | 0.66 | 0.35 | -0.37 | 0.11 | 0.10 |
| L | 1.29 | 1.35 | 0.02 | 0.37 | -1.39 | 0.98 | -0.23 | 0.46 | 1.53 | 0.71 | 0.82 | 1.12 | 0.51 | 1.09 | 1.70 | 1.37 | 1.06 | 0.34 | 0.82 | 0.81 |
| M | 0.84 | 0.90 | -0.43 | -0.08 | -1.83 | 0.53 | -0.67 | 0.02 | 1.08 | 0.26 | 0.37 | 0.67 | 0.06 | 0.64 | 1.25 | 0.93 | 0.61 | -0.11 | 0.37 | 0.36 |
| N | -5.75 | -5.69 | -7.02 | -6.67 | -8.43 | -6.06 | -7.26 | -6.58 | -5.51 | -6.33 | -6.22 | -5.92 | -6.53 | -5.95 | -5.34 | -5.66 | -5.98 | -6.70 | -6.22 | -6.23 |
| P | -1.15 | -1.09 | -2.42 | -2.07 | -3.83 | -1.46 | -2.67 | -1.98 | -0.91 | -1.73 | -1.62 | -1.32 | -1.93 | -1.35 | -0.74 | -1.07 | -1.38 | -2.10 | -1.62 | -1.63 |
| Q | -1.39 | -1.33 | -2.66 | -2.31 | -4.06 | -1.70 | -2.90 | -2.21 | -1.15 | -1.97 | -1.86 | -1.56 | -2.17 | -1.59 | -0.98 | -1.30 | -1.62 | -2.34 | -1.86 | -1.87 |
| R | 0.37 | 0.43 | -0.90 | -0.54 | -2.30 | 0.06 | -1.14 | -0.45 | 0.61 | -0.20 | -0.10 | 0.20 | -0.40 | 0.17 | 0.78 | 0.46 | 0.15 | -0.58 | -0.09 | -0.10 |
| S | -1.98 | -1.91 | -3.24 | -2.89 | -4.65 | -2.29 | -3.49 | -2.80 | -1.73 | -2.55 | -2.45 | -2.15 | -2.75 | -2.17 | -1.57 | -1.89 | -2.20 | -2.93 | -2.44 | -2.45 |
| T | -2.31 | -2.24 | -3.57 | -3.22 | -4.98 | -2.62 | -3.82 | -3.13 | -2.07 | -2.88 | -2.78 | -2.48 | -3.08 | -2.51 | -1.90 | -2.22 | -2.53 | -3.26 | -2.77 | -2.78 |
| V | 0.95 | 1.02 | -0.31 | 0.04 | -1.72 | 0.64 | -0.56 | 0.13 | 1.20 | 0.38 | 0.48 | 0.78 | 0.18 | 0.76 | 1.36 | 1.04 | 0.73 | 0.00 | 0.49 | 0.48 |
| W | 1.74 | 1.80 | 0.47 | 0.82 | -0.93 | 1.43 | 0.23 | 0.91 | 1.98 | 1.16 | 1.27 | 1.57 | 0.96 | 1.54 | 2.15 | 1.83 | 1.51 | 0.79 | 1.27 | 1.26 |
| Y | 2.12 | 2.19 | 0.86 | 1.21 | -0.55 | 1.82 | 0.61 | 1.30 | 2.37 | 1.55 | 1.65 | 1.95 | 1.35 | 1.93 | 2.54 | 2.21 | 1.90 | 1.18 | 1.66 | 1.65 |